Glycosylation and other post-translational modifications can be analyzed by LC-MS. LC-MS is a convenient method for identity testing using a reference standard and to determine purity of drug product or drug substance.
Plastics from pharmaceutical packaging or exposed to the drug substance or drug product during the manufacturing process could leach into the product and potentially cause toxic side effects. Likewise materials used in the production of medical device could also cause patient harm. Quality Control — LC-MS is a convenient method for identity testing using a reference standard and to determine purity of drug product or drug substance.
Extractable Leachable Studies — Plastics from pharmaceutical packaging or exposed to the drug substance or drug product during the manufacturing process could leach into the product and potentially cause toxic side effects. Pacific BioLabs. High-performance liquid chromatography is a popular separation process used in analytical chemistry to identify and quantify each compound in a sample mixture. Since it relied on pumps to flow a pressurized liquid solvent containing the sample combination through a column filled with a solid adsorbent Generally, silica material, this technique was previously known as high-pressure liquid chromatography.
The sample is injected through the injector, and the mobile phase flows it through the column. The solid adsorbent material interacts differently with each analyte in the sample mixture, resulting in different flow rates for various analytes. The separation and retention time depend on the affinity of the analyte towards the stationary phase. Mass spectrometry is used to determine the mass-to-charge ratio of charged particles, while liquid chromatography is a separation technique.
As a result, it has a wide range of applications, including pharmaceutical, environmental, research, and food analysis, etc. Combining the two analytical methods reduces experimental error and improves accuracy. The application of LC-MS is very useful in situations that involve a huge number of compounds, such as environmental effluents.
LC-MS involves separating mixtures in accordance with their physical and chemical properties, then identifying the components within each peak and detecting based on their mass spectrum. Therefore, the column in LC-MS is much smaller to accommodate the smaller solvent flow rates and sample volumes. This makes syringe pumps very convenient for LC-MS because they are very accurate and can deliver very low flow rates. In addition, it is possible to use syringe pumps for sample injection into the system as they can deliver very precise sample dosing.
Have any questions or comments about this article? Fill out the form below and we'll get back to you as soon as possible! Your Name required. Your Email required. Your Message. You Also agree to our Privacy Policy , which describes how we process your information. How LC Liquid Chromatography Works The device is a column packed with the porous medium made of a granular solid material i. The common types of pumping are: 1.
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